Composite
pT7+pHOA

Part:BBa_K784000:Experience

Designed by: Lior Levy   Group: iGEM12_Technion   (2012-09-13)
Revision as of 10:43, 17 September 2012 by Liorlevyzzz (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K784000

We have used the part inside a BL21 strain of E.coli which has an endogenouse PET system.
In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).
We checked the absorbance resulted from the PNP (para-nitrophenol phosphate) hydrolysis assay in raising concetrations of the inducer (IPTG) and the results are before you in the graph:

As you can see in the graph, raising concentrations of the inducer results in raising absorbance, which means raising concentration of the Alkaline Phosphatase enzyme.

User Reviews

UNIQ13d198b82c148f22-partinfo-00000000-QINU UNIQ13d198b82c148f22-partinfo-00000001-QINU