Composite
pSP6+phoA

Part:BBa_K784030

Designed by: Lior Levy   Group: iGEM12_Technion   (2012-09-17)
Revision as of 10:20, 17 September 2012 by Registry (Talk | contribs)

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pSP6+RBS+Alkaline Phosphatase (pHO)+Terminator

This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎ The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎ The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1506
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1506
    Illegal XhoI site found at 18
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1506
    Illegal NgoMIV site found at 345
    Illegal NgoMIV site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None