Part:BBa_K784023

MCS in pSB1C3

The sequence below has been created in order to expand the number of restriction sites available for shipping BioBricks in pSB1C3. If you want to ship a sequence that can't be digested with restriction sites found in the BioBrick prefix or suffix, this MCS can be the solution.

The addition of the MCS to the pSB1C3 backbone resulted in a change in the BioBrick prefix. This was in order to add the BglII restriction site in overlap to the XbaI restriction site from the prefix.

The restriction sites available in the MCS are: BglII, HindIII, PacI and BamHI. These restriction sites are preceded by the EcoRI, NotI and XbaI sites from the BioBrick prefix. They are also followed by the SpeI, NotI and PstI sites from the BioBrick suffix.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 22
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]