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Part:BBa_K784021:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-17)
Revision as of 07:00, 17 September 2012 by Registry (Talk | contribs)

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Phage Lambda fragment 7 - replication and lytic regulation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal BglII site found at 275
    Illegal BglII site found at 335
    Illegal BamHI site found at 3253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal AgeI site found at 1973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4236


Design Notes

Hila, update this.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References