Coding

Part:BBa_K929001:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-16)
Revision as of 06:22, 17 September 2012 by Rakku7 (Talk | contribs) (→‎Design Notes)

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AID without NES, with NLS and Kozak sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal SapI site found at 209


Design Notes

Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS:
ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG

Primer (reverse, complement)with AgeI, SpeI and PstI recognition site:
GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC

UP12 biobrick1.jpg

Source

AID BioBrick (BBa_K103001) and self-designed primers

References