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Part:BBa_K784020:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 16:46, 16 September 2012 by Registry (Talk | contribs)

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Phage Lambda fragment 6 - regulation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3822
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1500
    Illegal PstI site found at 3822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2532
    Illegal BglII site found at 4924
    Illegal BglII site found at 5575
    Illegal BglII site found at 5635
    Illegal BamHI site found at 1320
    Illegal XhoI site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3822
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1148
    Illegal SapI.rc site found at 1621


Design Notes

Hila, update this.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References