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Part:BBa_K784016:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 16:03, 16 September 2012 by Sl.ilya (Talk | contribs) (→‎Source)

Phage Lambda fragment 2 - tail proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
    Illegal AgeI site found at 2771
    Illegal AgeI site found at 3156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3645
    Illegal SapI.rc site found at 923
    Illegal SapI.rc site found at 2591
    Illegal SapI.rc site found at 5507


Design Notes

Hila, update this.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1
The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp

References