Generator

Part:BBa_K861161

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 06:09, 16 September 2012 by Shinkwee (Talk | contribs)

CRP with a RBS and a terminator

Coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcriptional dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of binding site in the CRP regulated promoter. This part contains CRP with a RBS and double terminators.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aCRP with a RBS and a terminator