Coding

Part:BBa_K748003

Designed by: Lei Qiao   Group: iGEM12_HIT-Harbin   (2012-09-15)
Revision as of 05:22, 16 September 2012 by Danran (Talk | contribs)

yddV is a Diguanylate Cyclase-Genomic.

yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3’-5’)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//cds/enzyme
//cds/enzyme/dnase
//chassis/prokaryote/ecoli
Parameters
None