Terminator
Part:BBa_K731722
Designed by: Anna Depetris, Giacomo Giacomelli Group: iGEM12_UNITN-Trento (2012-08-23)
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T1 terminator from E. coli rrnB
As many different teams had problems extracting BBa_B0010 from the Registry and cloning it, this is the same part newly submitted, confirmed by sequencing and by experience...ready to use! The characterization of this terminator has been done both with T7 and E. coli RNA polymerases using BBa_K731700 and BBa_K731710 by the 2012 Trento iGEM team. |
Examples of Secondary Structures
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Categories
Parameters
//rnap/bacteriophage/t7
//terminator/single
//terminator/single
biology | rrnBT1 |
direction | Forward |
forward_efficiency | 0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac) |