Composite

Part:BBa_K896003

Designed by: Hsun-I Chiu   Group: iGEM12_NYMU-Taipei   (2012-09-12)
Revision as of 03:55, 15 September 2012 by Cutelucypoop (Talk | contribs)

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Sulfide quinone reductase(SQR) on neutral site 2 vector 

 Introduction of coding sequence

The coding sequence, sulfide quinone reductase(SQR), cloned out from Synechococcus sp. PCC 7002 plasmid pAQ7. SQR could transfer electrons from sulfide into the quinone pool and trigger the sulfide-dependent photosynthesis, which is only based on photosystem I and shuttle the photosystem II down. Fully introduction and cloning of SQR gene is on BBa_K896000. The assembly compatibility of BBa_K896000 is EcoRI and XbaI on 5' end and SpeI and PstI on 3' end. 

 Introduction of vector

The vector(provided from Dr. Pei-Hong Chen) is specific to neutral site 2 for Synechococcus elongatus PCC7942. The neutral site 2 vector(4538 bp) consists of trc promoter(1718-1963 bp), multiple cloning site(1965-2014 bp), and Streptomycin resistence gene(2555-3343 bp). 

 Integration of SQR and neutral site 2 vector

Our SQR gene integrated into neutral site 2 vector on multiple cloning site

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 349


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