Plasmid_Backbone
pSac-Cm_BB

Part:BBa_K818000:Design

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-08-22)
Revision as of 12:55, 14 September 2012 by Emeraldo88 (Talk | contribs) (→‎Design Notes)

Integration vector for Bacillus subtilis derived from pSac-Cm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal BamHI site found at 180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5207
    Illegal XbaI site found at 5218
    Illegal SpeI site found at 1
    Illegal PstI site found at 12
    Illegal NgoMIV site found at 4124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2051
    Illegal SapI.rc site found at 3133


Design Notes

This part backbone plasmid was designed from psac-cm Bacillus subtilis backbone plasmid. It has

Source

MolGen Group (University of Groningen)

References