Composite
pT7+pHOA

Part:BBa_K784000

Designed by: Lior Levy   Group: iGEM12_Technion   (2012-09-13)
Revision as of 15:19, 13 September 2012 by Liorlevyzzz (Talk | contribs)

pT7+RBS+Alkaline Phosphatase (pHO)+Terminator

This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎
The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the following assay PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes. ‎


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1512
    Illegal XhoI site found at 24
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1512
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 798
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None