Coding

Part:BBa_K777000:Design

Designed by: Bianca Genenncher   Group: iGEM12_Goettingen   (2012-09-11)
Revision as of 18:35, 11 September 2012 by Registry (Talk | contribs)

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Tar receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.


Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).

References