Part:BBa_K823026

pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has an ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid).

This backbone is a BioBricked version of the B. subtilis vector pDG148.

This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph et al.]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 8274
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal BglII site found at 5862
  Illegal BamHI site found at 1378
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 8268
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 8268
  Plasmid lacks a suffix.
  Illegal XbaI site found at 8283
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2925
  Illegal AgeI site found at 5473
  Illegal AgeI site found at 6435
  Illegal AgeI site found at 7110
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2749
  Illegal BsaI.rc site found at 4188
  Illegal BsaI.rc site found at 6704
  Illegal SapI site found at 1666
  Illegal SapI.rc site found at 5686