Generator

Part:BBa_K861023

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-06)
Revision as of 06:03, 11 September 2012 by Shinkwee (Talk | contribs)

S-FadE with a RBS and a terminator

FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE have far better efficiency compared to FadE in E.coli. The original S-FadE gene contains PstI. This parts has already been point mutated (The G at 1983bp was mutated to A).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 378
    Illegal AgeI site found at 2568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535
    Illegal SapI.rc site found at 823
    Illegal SapI.rc site found at 1084


[edit]
Categories
Parameters
None