Tag

Part:BBa_K823037

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-09-08)
Revision as of 12:34, 8 September 2012 by Dexios (Talk | contribs)

10x His-tag (Freiburg standard+RBS)

10xHis-tag with RBS in Freiburg standard.


prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:

3x Flag - tag

HA - tag

cMyc - tag

His - tag

Strep - tag


Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
Parameters
None