Tag
Part:BBa_K823036
Designed by: Jara Radeck Group: iGEM12_LMU-Munich (2012-09-07)
cMyc-tag (Freiburg standard+RBS)
cMyc-tag with RBS in Freiburg standard.
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:
[Part:BBa_K823034|3x Flag tag],[Part:BBa_K823035|HA-tag],[Part:BBa_K823036|cMyc-tag],[Part:BBa_K823037|3x Flag tag],[Part:BBa_K823038|3x Flag tag]
See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 24
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//proteindomain/affinity
None |