Reporter

Part:BBa_K861176

Designed by: Kuanwei Sheng, Xian Xia   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 09:35, 8 September 2012 by Registry (Talk | contribs)

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mRFP expressed after Pcar

PII is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.Therefore due to the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter.This generator contains PII and a screening plasmid intermediate designed by Josh.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amRFP expressed after Pcar