Coding

Part:BBa_K883404

Designed by: Marnix Vlot   Group: iGEM12_Wageningen_UR   (2012-09-07)
Revision as of 11:24, 7 September 2012 by Eling006 (Talk | contribs)

PLRV Coat Protein gene - Isolated from Marfona potato

This part encodes the major coat protein of the Potato Leafroll Virus (PLRV). It was isolated from a PLRV infected Marfona potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR.


The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//direction/forward
Parameters
biologyPotato Leafroll Virus
proteinMajor Coat Protein