Generator

Part:BBa_K861101

Designed by: Xian Xia   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 07:13, 7 September 2012 by Registry (Talk | contribs)

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BcsA with RBS and terminator

BcsA, BcsB, BcsZ and BcsC are involved in biosynthesis of cellulose, a characteristic of the rdar morphotype. BcsA is considered to be the catalytic subunit for cellulose biosynthesis. The cellulose synthase is located in the cytoplasmic membrane with 8 to 10 predicted transmembrane domains.Besides the high homology to other processive glycosyltransferases, BcsA has been experimentally shown to bind the substrate UDP-glucose.This device contains BcsA with a RBS and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 873
    Illegal BamHI site found at 947
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 729


[edit]
Categories
Parameters
n/aBcsA with RBS and terminator