Composite
Part:BBa_K902007:Design
Designed by: Emily Hicks Group: iGEM12_Calgary (2012-09-06)
Beta-D-glucoside glucohydrolase (bglX) with strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1639
Illegal AgeI site found at 1861
Illegal AgeI site found at 2050 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An illegal pstI site was removed from the 15th and 16th amino acid positions via site directed mutagenesis.
Source
The beta-D-glucoside glucohydrolase (bglX) gene was amplified from TOP10 E. coli via polymerase chain reaction.