Plasmid_Backbone

Part:BBa_K268000:Experience

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)
Revision as of 22:30, 6 September 2012 by Earthnut (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K268000

User Reviews

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Earthnut

WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.

An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.3&nbbsp;kbp and one at 1.4 kbp.

TU_MUNICH 2012

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UNIQ5a66862c7182c20f-partinfo-00000001-QINU UNIQ5a66862c7182c20f-partinfo-00000002-QINU