Plasmid_Backbone

Part:BBa_K268000:Experience

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)
Revision as of 22:26, 6 September 2012 by Earthnut (Talk | contribs)

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WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.

An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.3&nbbsp;kbp and one at 1.4 kbp.


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UNIQ773ddf0ed5fd0d08-partinfo-00000001-QINU UNIQ773ddf0ed5fd0d08-partinfo-00000002-QINU