Terminator

Part:BBa_K731722

Designed by: Anna Depetris, Giacomo Giacomelli   Group: iGEM12_TRENTO   (2012-08-23)
Revision as of 10:49, 5 September 2012 by Giacomo (Talk | contribs)

Terminator.png

biologyrrnBT1
directionForward
forward_efficiency0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac)

T1 terminator from E. coli rrnB

We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.

Examples of Secondary Structures

B0010 1.png B0010 2.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
//rnap/bacteriophage/t7
//terminator/single
Parameters
biologyrrnBT1
directionForward
forward_efficiency0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac)