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Part:BBa_K763000

Designed by: Pedro Luis Dorado Morales   Group: iGEM12_Valencia_Biocampus   (2012-09-03)
Revision as of 11:04, 3 September 2012 by Registry (Talk | contribs)

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pLac + Gene encoding AmCyan

The promoter is activated when: (1) there is no glucose in the medium and (2) there is lactose in the medium. Our construction uses the well-known lactose operon system. A lack of glucose promotes the formation of CAP (or CRP), which binds to specific sites upstream of sugar-metabolization genes and activates its transcription. Moreover, another condition is needed since LacI repressor will bind to the operator region and will block the transcription unless lactose is also in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


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