Generator

Part:BBa_K819005:Design

Designed by: ZHANG Zidong   Group: iGEM12_Peking   (2012-09-02)
Revision as of 15:43, 2 September 2012 by Registry (Talk | contribs)

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Constitutive LuxBrick Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


Design Notes

LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and I135M enable the sensor recognize 408 form of SOS box and with enhanced performance.


Source

Construct by Peking2012.

References