Composite

Part:BBa_K731400:Design

Designed by: Andrea Tassinari   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 00:08, 31 August 2012 by Lmartini (Talk | contribs) (→‎Design Notes)

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

The nucleotide sequence of CysDes K731300 used to create this composite part has been codon optimised before Gene Synthesis by Genescript.

Source

K731300 and K731600

References