Regulatory

Part:BBa_K754000:Design

Designed by: iGEM Valencia 2012   Group: iGEM12_Valencia   (2012-08-28)
Revision as of 18:21, 29 August 2012 by Jesterface (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

S. elongatus PCC7942 psbAI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence (initially a guanine) had to be changed by an adenine in order to remove an XbaI target

Source

The sequence of this part comes from the AM 2195 plasmid developed by doctor Susan Golden.

References