Composite

Part:BBa_K741018

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-08-29)
Revision as of 06:22, 29 August 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T

The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1589
    Illegal NheI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1370
    Illegal BglII site found at 1706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2500


[edit]
Categories
Parameters
None