Plasmid

Part:BBa_K524204:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2012-08-26)
Revision as of 16:48, 26 August 2012 by Hyht2011 (Talk | contribs)

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pTE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lox or FRT sites were not included in this plasmid, partly because no qualified BioBricks were available (functionality of parts for lox66 and lox71 were questioned, and FRT sites were not available from DNA Kit Plates).


Source

The pTE plasmid was formerly the BBa_S05033 insert that resided in pSB1A2, which was excised out with XbaI and SpeI, and then self-ligated to generate the plasmid.

References

Datsenko KA, Wanner BL.(2000).One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5