DNA

Part:BBa_K524203:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2012-08-26)
Revision as of 15:09, 26 August 2012 by Hyht2011 (Talk | contribs) (→‎References)

pSC101 heat sensitive origin of replication with deletion in par locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then


Source

Modified from BBa_K524000

References

Tucker WT, Miller CA, Cohen SN. Structural and functional analysis of the par region of the pSC101 plasmid. Cell. 1984;38:191–201.