Terminator

Part:BBa_K731722:Design

Designed by: Anna Depetris, Giacomo Giacomelli   Group: iGEM12_TRENTO   (2012-08-23)
Revision as of 20:41, 23 August 2012 by Anna.d (Talk | contribs) (→‎Design Notes)

T1 terminator from E. coli rrnB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The terminator is in the standard backbone pSB1C3. Note: it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.

Source

This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.

References