Terminator
Part:BBa_K731722:Design
Designed by: Anna Depetris, Giacomo Giacomelli Group: iGEM12_TRENTO (2012-08-23)
T1 terminator from E. coli rrnB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
Source
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.