Coding

Part:BBa_K731600:Design

Designed by: Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli   Group: iGEM12_UNITN-Trento   (2012-08-23)
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Cysteine Desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nucleotide sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in Treponema denticola. The sequence of the lac operator corresponds to the sequence found in pET21b.


Source

CysDes nucleotide sequence was synthesized by Genscript and subcloned into psB1C3 to create part BBa_K731600.

References