Composite

Part:BBa_K731520

Designed by: Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)
Revision as of 15:34, 22 August 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


IPTG inducible GFPmut3b

This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.

lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1910


[edit]
Categories
Parameters
None