Regulatory
Part:BBa_K731500
Designed by: Francesco Guzzonato Group: iGEM12_UNITN-Trento (2012-08-22)
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
This is E.coli lactose-inducibile promoter.
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |