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Part:BBa_K731480:Design

Designed by: Andrea Tassinari, Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)
Revision as of 11:52, 22 August 2012 by Registry (Talk | contribs)

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IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2460
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1559
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2490


Design Notes

The sfGFP is the BioBrick BBa_K515005 designed by iGEM11_Imperial_College_London.


Source

LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.

References