Plasmid_Backbone
pSBBs3C

Part:BBa_K823025:Design

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-21)
Revision as of 10:49, 21 August 2012 by Registry (Talk | contribs)

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pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 10619
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 10625
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 10619
    Illegal BglII site found at 5170
    Illegal BamHI site found at 5705
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 10619
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 10619
    Plasmid lacks a suffix.
    Illegal XbaI site found at 10634
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 9536
    Illegal AgeI site found at 576
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 7576
    Illegal BsaI.rc site found at 3489
    Illegal SapI site found at 1095
    Illegal SapI.rc site found at 8658


Design Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.


Source

This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.

References