Composite
CysE-sfGFP

Part:BBa_K731040

Designed by: Jason Fontana, Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 08:45, 21 August 2012 by Jasonfweb (Talk | contribs)

Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

araC-pBAD regulating CysE M256 (K731030) was fused at his 3' with sfGFP (spaced by a GSGSGS linker) using Gibson's Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2075


[edit]
Categories
Parameters
None