Composite
araC-CysEm

Part:BBa_K731030:Design

Designed by: Jason Fontana, Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 08:30, 21 August 2012 by Jasonfweb (Talk | contribs) (→‎Source)

Inducible araC-pBAD promoter regulating M256I CysE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.

Source

K731200, K731010

References