Coding
M256I CysE

Part:BBa_K731010

Designed by: Jason Fontana, Daniele Rossetto, Giacomo Giacomelli   Group: iGEM12_UNITN-Trento   (2012-08-20)
Revision as of 16:21, 20 August 2012 by Registry (Talk | contribs)

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M256I CysE (Serine Acetyltransferase)

CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.

This mutated version results to be not inhibited by Cys overproduction, as it secretes Cys.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 698
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None