Coding

Part:BBa_K738004:Design

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-08-09)
Revision as of 09:38, 9 August 2012 by Registry (Talk | contribs)

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FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 835
    Illegal BsaI site found at 1219


Design Notes

This part should be expressed in e.coli BL21(DE3) because of its T7 promoter and terminator.


Source

Dr. Camille J. Delebecque, the author of Organization of Intracellular Reactions with Rationally Designed RNA Assemblies, offer us FA-2X-MS2 secquence.

References