Coding
Part:BBa_K738004
Designed by: Huachun Liu Group: iGEM12_ZJU-China (2012-08-09)
FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein
FA is half of split GFP with 2X linker[Gly4Ser]2, fusion expressed with MS2.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 835
Illegal BsaI site found at 1219
[edit]
Categories
Parameters
n/a | FA-2X-MS2: Half of split GFP |