Composite

Part:BBa_K741012

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-25)
Revision as of 05:00, 25 July 2012 by Registry (Talk | contribs)

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plac-anticro-T -pCon(0.856)-RBS-crogfp-T

The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
    Illegal BglII site found at 1481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918


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Categories
Parameters
None