Device

Part:BBa_J100080

Designed by: Rebecca Evans   Group: Campbell M Lab   (2012-07-07)
Revision as of 15:31, 19 July 2012 by Reevans (Talk | contribs)

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Riboswitch and GFP

This part combines Riboswitch E (Part J100066) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


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Categories
Parameters
None