Composite

Part:BBa_K727727:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)
Revision as of 15:55, 25 June 2012 by BioBrickMaster (Talk | contribs) (→‎Source)

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FMO3 Generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2669
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1053
    Illegal SpeI site found at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
    Illegal PstI site found at 2684
    Illegal NotI site found at 1059
    Illegal NotI site found at 2677
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1053
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1053
    Illegal XbaI site found at 1068
    Illegal SpeI site found at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
    Illegal PstI site found at 2684
    Illegal NgoMIV site found at 1936
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

Optimized for E.coli


Source

Genom of Human

References