Regulatory
precB

Part:BBa_K862003

Designed by: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)   Group: iGEM12_Heidelberg_LSL   (2012-06-08)
Revision as of 09:05, 8 June 2012 by Registry (Talk | contribs)

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precB

The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.

We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.

RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote
//chassis/prokaryote/ecoli
//function/sensor
Parameters
None