Coding
E

Part:BBa_K896999:Design

Designed by: Jesse Wu   Group: iGEM12_NYMU-Taipei   (2012-04-04)
Revision as of 04:20, 5 April 2012 by Blackrabbit (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

ΦX174; E (lysis) gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used in the PCR: 

X174_E_FP: gcttctag atggtacgctggactttgt (TM:55C)
X174_E_RP: gct ctgcagcggccgctactagta tcactccttccgcacg (TM:55C)

Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.

Source

PCR'd from ΦX174 wildtype phage.

References

[Zheng09] Zheng et al (2009) Purification and Functional Characterization of ΦX174 Lysis Protein E, Biochemistry, 48 (22), pp 4999–5006