Protein_Domain
PLrigid

Part:BBa_J176131

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-10-28)
Revision as of 21:48, 7 March 2012 by Kahaynes (Talk | contribs)

PLrigid

(EAAAR)4 peptide linker, rigid; alpha helix secondary structure


Usage and Biology

PLrigid is a short peptide that has an alpha-helix structure. We use it as a "linker" to connect protein domains/ modules. Alpha helices are thought to be rigid structures that restrict the position of one end relative to the other. Lack of "bendability" keeps the two proteins attached at either end from coming closer together. Since the proteins are also unable to move farther apart when they are attached with a rigid linker, the relative position of the two proteins is fixed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/linker
Parameters
functionprotein linker